A new strain Acidithiobacillus albertensis BY-05
for bioleaching of metal sulfides ores
for bioleaching of metal sulfides ores
(Key laboratory of Biometallurgy of Ministry of Education, School of Minerals Processing and Bioengineering,
Central South University, Changsha 410083, China)
Central South University, Changsha 410083, China)
Abstract: An acidophilic, rod-shaped Gram-negative sulfur oxidizing strain BY-05 was isolated from an acid mine drainage of copper ore in Baiyin area, Gansu Province, China. Ultrastructural studies show that the isolate has a tuft of polar flagella and possesses sulfur granules with clear membrane adhering to the cell innermembrane. Physiological study shows that this isolate grows autotrophically and aerobically by oxidizing S0 and reduced inorganic sulfur compounds ( S2O2-3, S2O2-4, S2− and ZnS) with the optimum growth at pH 3.5−4.0 and at the temperature range of 25−30 ℃. The 16S rRNA gene sequence (DQ 423683) of strain BY-05 has 100% sequence similarity to that of Acidithiobacillus albertensis (DSM 14366). So it is identified and named as A. albertensis BY-05. Bioleaching experiments with this new strain show that it can play an important role in recovery of metals from chalcopyrite and sphalerite.
Key words: sulfur oxidation; Acidithiobacillus albertensis; Acidithiobacillus thiooxidans; isolation and characterization of bacteria; bioleaching