Preparation of poly-L-lactide/bioactive glass composite and evaluation of cytotoxicity in vitro
(1. State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China;
2. College of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China;
3. Department of Orthopedics, the Second Xiangya Hospital, Central South University, Changsha 410011, China)
2. College of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China;
3. Department of Orthopedics, the Second Xiangya Hospital, Central South University, Changsha 410011, China)
Abstract: Bioactive and bioresorbable composite materials were fabricated from poly-L-lactide and bioactive glass (average particle size 6.8 μm) by a solvent evaporation technique. Cellular cultivation in vitro and MTT assay were conducted for evaluating the influence on morphology, growth and proliferation of cultured fibroblasts. The results of cytotoxicity testing show that cells cultured in extracts of PLLA/BG and on the surface of composites demonstrate normal growth and proliferation. The bioactive glass in PLLA composite facilitates both adhesion and proliferation of rat fibroblasts on PLLA/bioactive glass composite film.
Key words: poly-L-lactide; bioactive glass; composite; cytotoxicity; biocompatibility; in vitro