Liquid-nitrogen cryopreservation of three kinds of
autotrophicbioleaching bacteria
autotrophicbioleaching bacteria
(1. Key Laboratory of Biometallurgy of Ministry of Education,
School of Minerals Processing and Bioengineering, Central South University,
Changsha 410083, China;
2. China Center for Type Culture Collection, College of Life Science, Wuhan University,
Wuhan 430072, China)
School of Minerals Processing and Bioengineering, Central South University,
Changsha 410083, China;
2. China Center for Type Culture Collection, College of Life Science, Wuhan University,
Wuhan 430072, China)
Abstract: Three kinds of autotrophic bioleaching bacteria strains, including mesophilic and acidophilic ferrous ion-oxidizing bacteria Acidithiobacillus ferrooxidans (A. ferrooxidans), mesophilic and acidophilic sulfur-oxidizing bacteria Acidithiobacillus thiooxidans (A. thiooxidans), and moderately thermophilic sulfur-oxidizing bacteria Acidianus brierleyi, were cryopreserved in liquid nitrogen and their ferrous ion- or sulfur-oxidizing activities were investigated and compared with the original ones. The results revealed that ferrous ion/sulfur oxidation activities of the strains were almost equal before and after cryopreservation. Glycerin was used as cryoprotective agent. In conclusion, liquid-nitrogen cryopreservation is a simple and effective method for autotrophic bioleaching microorganisms.
Key words: A. ferrooxidans; A. thiooxidans; Acidianus brierleyi; cryopreservation; liquid nitrogen; growth activities