A new strain Leptospirillum ferriphilum YTW315 for bioleaching of
metal sulfides ores
metal sulfides ores
(1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;
2. Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha 410083, China)
2. Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha 410083, China)
Abstract: A new strain named YTW315 was isolated from Dexing area using the double-layer culture technique. The morphological, biochemical and physiological characteristics of YTW315 were studied. Physiological investigation indicates that the strain YTW315 is a strict (obligate) chemolithoautotroph, metabolizing ferrous iron and pyrite. The optimal growth conditions for the strain are 40 ℃ and pH 1.6. A phylogenetic analysis based on 16S rRNA sequences shows that the isolate is clustered to Leptospirillum ferriphilum with 99.8% similarity to Leptospirillum ferriphilum strain Fairview and ATCC 49881. The molar fraction of DNA (G+C) of the isolate is 58.1%. The strain can tolerate high concentration of Fe(Ⅲ) and As(V) (500 mmol/L and 50 mmol/L, respectively). Bioleaching experiment indicates that the strain can oxidize Fe(Ⅱ) efficiently, and after 30 d, 44.56% of copper and 95.31% of iron are extracted from chalcopyrite and pyrite, respectively.
Key words: Leptospirillum ferriphilum; bioleaching; isolation; characterization; phylogenetic analysis