Binding tendency with oligonucleotides and cell toxicity of
cetyltrimethyl ammonium bromide-coated single-walled carbon nanotubes
cetyltrimethyl ammonium bromide-coated single-walled carbon nanotubes
(1. State Key Laboratory for Powder Metallurgy, Central South University, Changsha 410083, China;
2. The Third Xiangya Hospital, Central South University, Changsha 410013, China;
3. Xiangya Medical School, Central South University, Changsha 410013, China)
2. The Third Xiangya Hospital, Central South University, Changsha 410013, China;
3. Xiangya Medical School, Central South University, Changsha 410013, China)
Abstract: Functionalized carbon nanotubes (CNTs) were made for the delivery of genes and drugs and CNT-based biosensors. The basis of CNTs is for binding with biomolecules in biomedical applications. The binding tendency with small interfering RNA oligonucleotides and cytotoxicity of cetyltrimethyl ammonium bromide (CTAB)-coated single-walled carbon nanotubes (SWNTs) were studied. The field emission scanning electron microscopy and transmission electron microscopy results show that a SWNT suspension in CTAB solution was well-dispersed and stable. CTAB is the cross-linker between SWNTs and oligonucleotides. The CTAB-coated SWNTs have less cytotoxicity to human umbilical vein endothelial cells than single SWNTs and the cytotoxicity of CTAB-coated SWNTs depended on the concentration of CTAB-coated SWNTs.
Key words: single-walled carbon nanotubes; cetyltrimethyl ammonium bromide; oligonucleotides; cytotoxicity