In vitro and in vivo evaluation of effects of Mg-6Zn alloy ontight junction of intestinal epithelial cell
(1. Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital,
Shanghai 200233, China;
2. School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China;
3. Department of General Surgery, Nanjing Medical University Affiliated Wuxi No. 2 People’s Hospital,
Nanjing 214002, China;
4. State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering,
Shanghai Jiao Tong University, Shanghai 200240, China;
5. Suzhou Origin Medical Technology Co. Ltd., Suzhou 215513, China)
Shanghai 200233, China;
2. School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China;
3. Department of General Surgery, Nanjing Medical University Affiliated Wuxi No. 2 People’s Hospital,
Nanjing 214002, China;
4. State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering,
Shanghai Jiao Tong University, Shanghai 200240, China;
5. Suzhou Origin Medical Technology Co. Ltd., Suzhou 215513, China)
Abstract: The effects of biodegradable Mg-6Zn alloy on tight junction of intestinal epithelial cells (IEC-6) were investigated. In the in vitro experiments, the cells were exposed to Mg-6Zn alloy extracts with different concentrations (0, 20% and 40%) for 1, 3 and 5 d. The real-time polymerase chain reaction (PCR) results show that when the cells are treated with 40% and 20% extracts, the expression of Zona Occludens 1 (ZO-1) and Occludin increase as compared with those in the control group. In the in vivo experiments, Mg-6Zn alloy and titanium staples were implanted into rabbits’ intestinal tract for 1, 2 and 3 weeks. By immunohistochemical staining of peri-implant intestinal tissue, increased expression of Occludin and ZO-1 are observed in the Mg-6Zn alloy groups as compared with those in the titanium and control groups. The results show that Mg-6Zn alloy in intestine may promote the regeneration of tight junction, and the extract with a certain concentration can induce the expression of tight junction related genes in IEC-6 cells.
Key words: Mg-6Zn alloy; intestinal epithelial cell; tight junction