Synthesis and in-vitro characterization of biodegradable porous magnesium-based scaffolds containing silver for bone tissue engineering
(1. Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran;
2. Advanced Membrane Technology Research Center (AMTEC), Universiti Teknologi Malaysia, 81310 Skudai, Johor Bahru, Johor, Malaysia;
3. Department of Mechanical, Materials and Manufacturing Engineering, University of Nottingham Malaysia Campus, 43500 Semenyih, Malaysia;
4. Division of Biomedical Engineering, College of Engineering, University of Saskatchewan, Saskatoon, SK, Canada;
5. Department of Mechanical Engineering, College of Engineering, University of Saskatchewan, Saskatoon, SK, Canada)
2. Advanced Membrane Technology Research Center (AMTEC), Universiti Teknologi Malaysia, 81310 Skudai, Johor Bahru, Johor, Malaysia;
3. Department of Mechanical, Materials and Manufacturing Engineering, University of Nottingham Malaysia Campus, 43500 Semenyih, Malaysia;
4. Division of Biomedical Engineering, College of Engineering, University of Saskatchewan, Saskatoon, SK, Canada;
5. Department of Mechanical Engineering, College of Engineering, University of Saskatchewan, Saskatoon, SK, Canada)
Abstract: Infection is a major potential complication in the clinical treatment of bone injuries. Magnesium (Mg)-based composites are biodegradable and antibacterial biomaterials that have been employed to reduce infection following surgical implants. The aim of present study was to synthesize and in-vitro characterize Mg-based scaffolds containing silver for bone tissue engineering. Porous Mg-based scaffolds with four silver concentrations (i.e., 0, 0.5 wt.%, 1 wt.%, and 2 wt.%), denoted by Mg-Ca-Mn-Zn-xAg (MCMZ-xAg) (where x is the silver concentration), were fabricated by the space holder technique. The effects of silver concentration on pore architecture, mechanical properties, bioactivity, and zone of bacterial inhibition were investigated in-vitro. X-ray diffractometry (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and fluorescence microscopy were utilized to characterize the obtained scaffolds. In-vitro corrosion test results indicated that the MCMZ scaffolds with lower silver content were more resistant to corrosion than those enriched with higher amounts of silver. Examination of the antibacterial activity showed that the MCMZ-Ag scaffolds exhibited superb potential with respect to suppressing the growth of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), in the inhibition zone around the MCMZ-Ag scaffolds, with increasing in the amount of incorporated silver; however, higher amounts of silver increased the cytotoxicity. Taken together, the results of this study demonstrate that the porous 0.5 wt.% Ag-containing scaffolds with interconnected pores, adequate mechanical properties, antibacterial activity, and cell adhesion are promising with respect to the repair and substitution of damaged and diseased bones.
Key words: Mg-based scaffold; biocompatibility; antibacterial activities; bioactivity; corrosion behavior