Responses of bacterial community to potential heap construction methods of fine-grained copper tailings in column bioleaching system
(1. Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection, College of Resources and Environment, Linyi University, Linyi 276000, China;
2. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China)
2. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China)
Abstract: The column bioleaching of copper flotation tailings was comparatively investigated using layered heap construction method (LM), agglomerate heap construction method (AM), and pellets-sintering heap construction method (PM). The bacterial communities of free, attached, weakly-attached, and strongly-attached microbes in the later bioleaching stage were investigated. In AM group, the addition of lump sulphide ore resulted in the low leachate pH, high ferric iron concentration, and rapid microbial adsorption, which obtained the maximum copper extraction (60.1%) compared with LM (54.6%) and PM (43.9%) groups. The relative abundance of dominant genera and microbial communities of different microbiota underwent changes in three heap construction methods. The alpha-diversity indexes of attached, weakly-attached, and strongly-attached microbes were different, while no significant change was observed in free bacteria. The variation of whole bacterial community was significantly associated with solution pH, total iron, and ferric iron concentrations. Pearson correlation analysis and partial least square path model both indicated that attached bacteria made larger contribution to the copper extraction of tailings.
Key words: fine-grained tailings; column bioleaching; heap construction method; free and attached bacteria; microbial diversity