The cyclic rotating-bending (CRB) processes under different deformation conditions were carried out to refine the microstructure and improve the mechanical properties of the 65Cu-35Zn brass tubes. The microstructure and the mechanical properties in the axial direction of the tubes after the CRB process were studied with the OM, EBSD and conventional tensile test. To obtain the accumulated effective plastic strain of the tube during the CRB process, the FEM simulation was also executed. The results show that the average grain size decreases with the increase of rotation time at RT, and with the decrease of bending angle at 200 °C. With the increase of accumulated effective plastic strain during the CRB process, the reduction rate of average grain size of the brass tube increases, the tensile strength of the brass tube increases in wave shape and the elongation increases first and then sharply decreases.