The corrosion resistance and antibacterial properties of Ti-3Cu alloy prepared by selective laser melting were evaluated using electrochemical experiments and a variety of antibacterial characterization. It is found that the charge transfer resistance of Ti-3Cu alloy was 4.89×10⁵ Ω？cm², which was doubled the data obtained by CP-Ti alloy. The antibacterial rates of Ti-3Cu alloy against S. mutans and P. gingivalis were 45.0% and 54.5%. And the antibacterial rates increased with the prolongation of cultivation time, reaching up to 62.8% and 68.6%, respectively. The in-situ nano Ti₂Cu precipitates were homogeneously distributed in the matrix of the Ti-3Cu alloy, which was the key reason of increasing the corrosion resistance. Additionally, the microscale electric fields between the α-Ti matrix and the Ti₂Cu was responsible for the enhancement of the antibacterial properties.